Isolation and preliminary study of one strain of Klebsiella pneumoniae phage

Abstract

Objective: To isolate and conduct a preliminary characterization of a bacteriophage strain targeting Klebsiella pneumoniae, aiming to explore its potential as an alternative treatment for antibiotic-resistant infections.
Methods: Environmental samples were collected and subjected to phage enrichment and isolation using Klebsiella pneumoniae as the host bacterium. The isolated phage was purified and examined for morphology via electron microscopy. Its lytic activity, host range, and stability under various conditions (pH, temperature) were preliminarily assessed.
Results: A novel phage strain specific to Klebsiella pneumoniae was successfully isolated. Electron microscopy revealed its morphology consistent with the Podoviridae or Myoviridae family (specify if known). The phage exhibited strong lytic activity against multiple clinical isolates of K. pneumoniae and maintained stability across a wide pH and temperature range.
Conclusion: The isolated Klebsiella pneumoniae phage shows promising characteristics as a potential biocontrol agent against antibiotic-resistant K. pneumoniae infections. Further detailed studies are warranted to evaluate its therapeutic application.