PCR-RFLP identification of two cases of Ancylostoma ceylanicum in Qinghai Province

Abstract

Objective:
To identify and confirm the presence of Ancylostoma ceylanicum in clinical cases from Qinghai Province using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique, providing molecular evidence for diagnosis and epidemiological study.
Methods:
Two suspected hookworm infection cases were investigated. DNA was extracted from stool samples and subjected to PCR amplification targeting specific gene regions of Ancylostoma species. The PCR products were digested with restriction enzymes, and the resulting fragment patterns were analyzed to differentiate A. ceylanicum from other hookworm species.
Results:
PCR-RFLP analysis successfully identified Ancylostoma ceylanicum in both cases. The restriction patterns matched the known profiles of A. ceylanicum, confirming the diagnosis. This represents one of the first molecular confirmations of A. ceylanicum infection in Qinghai Province.
Conclusion:
PCR-RFLP is an effective and reliable method for the molecular identification of Ancylostoma ceylanicum. The detection of these cases highlights the presence of this zoonotic hookworm in Qinghai Province, underscoring the need for increased surveillance and control measures.