Application of melting curve method to detect drug resistance of Mycobacterium tuberculosis in Tangshan Area

Abstract

Abstract
This study explores the application of the melting curve method to detect drug resistance in Mycobacterium tuberculosis strains isolated from patients in the Tangshan area. The melting curve analysis, a rapid molecular technique, was utilized to identify mutations associated with resistance to first-line anti-tuberculosis drugs. Clinical isolates were tested, and results were compared with conventional drug susceptibility testing to evaluate accuracy and efficiency. Findings demonstrate that the melting curve method provides a reliable, rapid, and sensitive approach for detecting drug-resistant tuberculosis, enabling timely adjustment of treatment regimens. This method shows promise for widespread use in clinical laboratories in Tangshan to improve the management of tuberculosis and curb the spread of resistant strains.