A review of isolation technology for Yersinia pestis phage

Abstract

Abstract
This review summarizes current advances in the isolation technologies of bacteriophages targeting Yersinia pestis, the causative agent of plague. Various isolation methods, including enrichment culture, plaque assay, and molecular detection techniques, are discussed with regard to their efficiency, specificity, and applicability. The review highlights challenges such as low phage abundance in environmental samples and the need for rapid, sensitive isolation approaches. Advances in genomics and bioinformatics have facilitated the identification and characterization of Y. pestis phages, contributing to phage therapy development and epidemiological studies. Future directions include optimizing isolation protocols and integrating novel technologies to improve phage recovery and application. This review provides valuable insights for researchers engaged in plague control and bacteriophage research.